Effect of Vitamin C and Aqueous Crude Extract of Aloe Vacillans (Audhalica) Leaves on the Hepatotoxicity
Introduction: This study was designed to evaluate the effects of vitamin C and aqueous extract of Aloe vacillans leaves on carbon tetrachloride (CCl4) - induced hepatotxicity in rabbits.
Methods: Hepatotoxicity was induced in rabbits by intraperitoneal injection of CCl4 in the olive oil 1:1(v/v) at dose l ml/kg on day 13 and 14. Vitamin C was administrated at dose 0.02 gm/kg of body weight and the aqueous crude extract of Aloe vacillans leaves were administrated at dose 500 mg/kg of body weight pass orally (p.o) daily for 15 days. The hepatotoxicity and its prevention were assessed by serum parameters like alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (T.B) and total protein (T.P).
Results: In CCl4 treated rabbits, significant increase in ALT, AST, bilirubin and decrease in the total T.P levels were shown (p<0.05), due to liver damage, when compared with normal group. Treatment with the aqueous extract of Aloe vacillans could significantly decrease the ALT, AST and T.B, increased T.P in serum at (p<0.05) when compared with CCl4 –treated group. Treatment with vitamin C could significantly decrease the AST), ALT and T.B, increased T.P in serum at p<0.05 when compared with CCl4 –treated group.
Conclusion: The data concluded that oral administration of aqueous extract of the leaves of Aloe vacillans and vitamin C significantly decreases the intensity of hepatic damage induced by CCl4 in rabbits when compared with all CCl4 –treated groups.
Keywords: Carbon tetrachloride, Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Total Protein, Total Bilirubin, Hepatic Damage, Rabbits.